144-147 Garcia LAJP 1864:Garcia

Rabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H+/K+ ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantification of RAB in pharmaceutical formulation and compare it with a capillary electrophoresis (CE) one, previously validated. The UV technique was applied using water (pH 10.0) as diluent and the determinations were made at λ = 291 nm. The method showed good linearity (r = 0.9997) in the concentration range of 6.0 to 18.0 μg ml–1. The intraand interday precision data demonstrated the method has good repeatability (RSD = 0.52 and 0.82, respectively). Accuracy and specificity were also evaluated and results were satisfactory. The detection and quantitation limits were 0.32 and 0.95 μg ml–1, respectively. Both methods demonstrated to be adequate for the intended purpose.